The findings from the present study suggest that the essential oils of lemongrass have potential in cancer prevention. Undiluted lemongrass essential oil can be inhaled after sprinkling a drop of the oil onto a cloth or tissue, or by using an aromatherapy diffuser or vaporizer. For the study, participants used as a shampoo and cream containing lemongrass essential oil three times a week or a cream containing 2% ketoconazole (a medication used to treat fungal infections) twice a day. Code of federal regulations-title 21, part 182-substances generally recognized as safe. The inlet temperature was 240°C and the oven temperature program was as follows: 60°C to 220°C at 4°C/min and then at 20°C/min to 240°C with an interphase temperature of 280°C. To evaluate the cytotoxic activities of the C. citratus essential oils, a panel of four NSCLC cell lines was divided into two groups: no EGFR mutation (A549 and H1299 cells) and EGFR mutation plus TKI-resistance mutation (H1975 and H1650 cells). Inhaling the aroma of lemongrass essential oil or absorbing it through the skin is said to transmit messages to the brain's limbic system, a region that influences the nervous system. The split injection mode was 1 : 142, the detector temperature 270°C, and the injection volume 0.1 μL. The HLL induced apoptosis and cycle arrest in A549 cells according to flow cytometric analysis and fluorescent nuclear staining assays. 1) [44, 45]. Thank you, {{form.email}}, for signing up. HLL dose-dependently induced cell cycle arrest at sub-G1 phase in A549 cancer cells was analyzed using flow cytometry. Skin Cafe 100% Natural Essential Oil – Lemongrass 10ml. Investigation of the mechanisms underlying the gastroprotective effect of cymbopogon citratus essential oil. Sign up here as a reviewer to help fast-track new submissions. J Young Pharm. A549 cells were seeded in a 6-well plate at a density of 2.5 × 105 cells/mL for 24 h and then treated with or without essential oils. Avoid applying it near the mouth, nose, and eyes. Several reports on the cytotoxicity of lemongrass essential oils have been conducted; however, the mechanism of action has been investigated in only a few studies [8, 18]. In previous studies, the essential oil of C. citratus was shown to exert various pharmacological activities, including antimicrobial [6], insecticidal [7], and cytotoxic activities [8, 9]. Briefly, cells were treated with various concentrations of essential oils and incubated for 48 h. The cells were then collected by trypsinization, washed 2 times with cold PBS, suspended in 100 μL of a binding buffer (diluted from 10x binding buffer), and stained with 5 μL PI (50 μg/mL stock solution) and 5 μL FITC-labeled Annexin-V in the dark for 15 min at room temperature. Can lemongrass essential oil be used in cooking?Yes. Fernandes C, De souza H, De oliveria G, Costa J, Kerntopf M, Campos A. Notably, camphene, valerianol, and epi-α-muurolol were found only in culm oil but not in leaf oil. Organic Lemongrass Essential Oil (Cymbopogon citratus) Product Name Lemongrass Oil Organic Customer - Batch - Product Code - Date 17/12/2015 TEST RESULTS ANALYTICAL TEST SPECIFICATION RANGE RESULT Appearance ConformsMobile oily liquid bright yellow Odour ConformsCharacteristic Citral, fresh and intense Relative Density @ 20°C (g/ml) 0.869 – 0.896 0.889 Optical Rotation @ 20°C … Tangpu V, Yadav A. Antidiarrhoeal activity of cymbopogon citratus and its main constituent citral. 2013;88(3):381-5. doi:10.1590/abd1806-4841.20131800, Goes T, Ursulino F, Almeida-Souza T, Alves P, Teixeira-Silva F. Effect of lemongrass aroma on experimental anxiety in humans. All essential oils exhibited cytotoxicity in the tested cells. The cells were analyzed by Novocyte 2000 (ACEA Biosciences Inc, USA). The obtained oils were stored at −5°C until use. The stained cells were observed using a fluorescence microscope (CKX53, Olympus, Tokyo, Japan) for determining any nuclear structural changes and apoptotic bodies. U.S. Food And Drug Administration. 2011;2(1):3-8. doi:10.4103/2231-4040.79796, Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W. Essential oils' chemical characterization and investigation of some biological activities: a critical review. The data used to support the findings of this study are included within the article. Lemongrass (Cymbopogon citratus, Cymbopogon flexuosus) oil is a type of essential oil often used in aromatherapy and by natural medicine practitioners for a variety of purposes, including reducing inflammation, relieving headaches, improving dandruff, and easing indigestion. According to the World Health Organization, more than 80% of the world’s population relies on folk medicine for their primary healthcare needs. Aromatherapy. A549 cells treated with HLL at concentrations 0, 1, and 10. Mix into a … We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. The Kim Duc culm (KDC) essential oil exerted the strongest cytotoxic effects against H1650 cells, with an IC50 value of 4.86 ± 0.29 μg/mL. 2011;2011:467134. doi:10.1093/ecam/nep213. Based on this observation, citral and geraniol may contribute to the cytotoxic activities of C. citratus essential oils. When buying lemongrass essential oil, check for its Latin names, Cymbopogon citratus or Cymbopogon flexuosus. The western blot analysis indicated that HLL induced the apoptotic effect by altering the regulating proteins of the apoptosis process such as caspase-3, Bcl-2, and Bax. The citral content was higher in the essential oil obtained from the leaf than in that from the culm of lemongrass in all regions. An ethanol extract of lemongrass exhibited significant anticancer properties in lymphoma and leukemia models and effectively induced apoptosis in a recent study [19]. Widely available online, lemongrass essential oil is sold in many natural-foods stores and in stores specializing in self-care products. doi:10.7860/JCDR/2015/14465.6581, De souza M, Vieira A, Beserra F, Pellizzon C, Nóbrega R, Rozza A. Gastroprotective effect of limonene in rats: influence on oxidative stress, inflammation and gene expression. Does lemongrass essential oil lighten skin? Cymbopogon citratus, popularly known as lemongrass, is used for the treatment of gastric, nervous and hypertensive disorders, in addition to its use in the food and pharmaceutical industries. HLL-treated A549 cells demonstrated a significant decrease in the expression level of Bcl-2 and a marked increase in the Bax level. Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation. Aromatherapy: do essential oils really work?. Duong Thu Trang, Thi Kim Van Hoang, Thi Thu Minh Nguyen, Pham Van Cuong, Nguyen Hai Dang, Hong Duyen Dang, Trung Nguyen Quang, Nguyen Tien Dat, "Essential Oils of Lemongrass (Cymbopogon citratus Stapf) Induces Apoptosis and Cell Cycle Arrest in A549 Lung Cancer Cells", BioMed Research International, vol. In Vietnam, 22,000 people are diagnosed with lung cancer and 19,500 die from the disease yearly. (lemongrass), belonging to the genus Cymbopogon, is widely used spices in tropical countries, especially in Southeast Asia. Although the composition of C. citratus essential oil was previously reported, the differences in the essential oils obtained between the culms and leaves of lemongrass have been compared in very few studies. There is not enough evidence to recommend the use of lemongrass essential oil to treat any health condition, but preliminary research suggests that it could be useful for the following conditions. Myrcene was the second abundant component in the C. citratus essential oils. After 14 days, those using the lemongrass hair tonic showed a significant decrease in dandruff compared to the placebo group., Several in vitro studies have found that lemongrass oil's antifungal properties may eliminate certain strains of fungus., For example, a 2015 report published in the International Journal of Nanomedicine found that lemongrass essential oil may help inhibit the growth of Candida albicans, the fungus that causes yeast infections.. Biochim Open. Her work is regularly featured in media such as First For Women, Woman's World, and Natural Health. J Clin Diagn Res. Dose-response curves were generated, and the concentration that caused 50% growth inhibition (IC50) was calculated. 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